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| Registros recuperados: 225 | |
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| Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S. |
| As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist... |
| Tipo: Text |
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf |
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| Gervais, Ophelie; Chollet, Bruno; Renault, Tristan; Arzul, Isabelle. |
| The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses.... |
| Tipo: Text |
Palavras-chave: Apoptosis; Host-parasite interactions; Parasite; Oyster; Bonamia ostreae; Ostrea edulis. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00346/45692/45312.pdf |
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| Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan. |
| Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely... |
| Tipo: Text |
Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf |
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| Harrang, Estelle; Bierne, Nicolas; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie. |
| L'huître plate européenne Ostrea edulis a connu une période faste dans l'aquaculture jusqu'à la fin des années 1960, lorsque deux parasites, Marteilia refringens (1969) et Bonamia ostreae (1979), ont successivement décimé les bancs naturels de cette espèce endémique, déjà affaiblis par la sur-exploitation de ses gisements. Historiquement présente sur toute la côte européenne, de la Mer du Nord à la Mer Méditerranée, en passant par l'Océan Atlantique, la Mer Adriatique et la Mer Noire, les bancs naturels de cette espèce sont devenus très localisés, laissant penser à des phénomènes d'adaptation locale de l'huître vis à vis de son environnement biotique et abiotique. La diversité et la structuration génétique de plusieurs populations de l'espèce, à l'échelle... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Sélection Génétique. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12547/9417.pdf |
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| Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre. |
| The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual... |
| Tipo: Text |
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf |
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| Robert, René; Borel, Michel; Pichot, Yves; Trut, Gilles. |
| A study of the growth and mortality of the European oyster Ostrea edulis , including the effects of Marteilia refringens and Bonamia ostreae , was carried out in three oceanic areas in the Bay of Arcachon from May 1989 to February 1991. One-year-old spat, free from disease were used for the trials. Six months after beginning the trials, Marteilia refringens had severely infested the cultures. Although Bonamia ostreae was found during the first year, the rate of infestation was low. In the second year bonamiasis increased and high mortality (> 60%) occurred in Ostrea edulis populations. Although the growth of the oysters was good, their condition index was generally poor. Moreover, shell perforations by Polydora sp. were observed. The influence of... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Pathologie; Mortalité; Croissance; Reproduction; Composition biochimique; Bassin Arcachon; Ostrea edulis; Pathology; Mortality; Growth; Reproduction; Gross biochemical composition; Bay of Arcachon. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00015/12600/9477.pdf |
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| Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre. |
| The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors. |
| Tipo: Text |
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf |
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| Buestel, Dominique; Ropert, Michel; Prou, Jean; Goulletquer, Philippe. |
| The history of French oyster culture consists of a succession of developmental phases using different species, followed by collapses caused by diseases. The indigenous species Ostrea edulis was replaced first with Crassostrea angulata, then C. gigas. France is now the top producer and consumer of oysters in Europe, producing around 120,000 t of the cupped oyster C. gigas annually, and an additional 1500 t of the flat oyster O. edulis. Cupped oysters are produced all along the French coast from natural and hatchery spat. Various structures are used to collect spat from the wild. After a growing-on period, oysters are Cultivated by three main methods: (1) on-bottom culture in the intertidal zone or in deep water, (2) off-bottom culture in plastic mesh bags... |
| Tipo: Text |
Palavras-chave: Cupped oyster; Flat oyster; Ostrea edulis; Crassostrea gigas; French production; Aquaculture. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7396.pdf |
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| Chupeau, Gaelle. |
| Le but de ce travail était de rechercher des marqueurs non neutres afin de comparer ces données obtenues avec celles données par les marqueurs précédemment étudiés (marqueurs neutres ou supposés l'être à savoir microsatellites, enzymatique et mitochondriales). Dû à des soucis d'ADN extrait des populations qui avaient été étudiées jusqu'alors, il a fallu effectuer un nouvel échantillonnage de l'huître plate Européenne, Ostrea edulis, sur son aire de répartition. Nous avons donc analysé en plus du but majeur, les données microsatellites pour ces nouvelles populations de manière à avoir un jeu de données de marqueurs non neutres. Ces analyses ont ainsi complété l'arbre phylogénétique déjà existant, et ont confirmé dans une moindre mesure puisque tous les... |
| Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Génétique des populations; Nucleotide Polymorphisme (SNP); Test de neutralisté; Microsatellites. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00033/14417/11712.pdf |
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| Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
| Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
| Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
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| Grizel, Henri. |
| Two successive diseases struck the flat oyster Ostrea edulis farms in Brittany. These two epizooties lead to some changes in the farming practices. The oyster culture developed towards the open ocean, in the sublitoral part. New techniques have been developed for the culture and collection. The flat oyster has been partially replaced by the cupped oyster Crassostrea gigas whose production went from 3 000 to 16 000 tonnes. They also had heavy consequences on the regional economy. The important mortalities decimated the stocks. The production dropped by about 17 000 tonnes from 1910 to 1982. For the same period of time, profits from the sale of the produced flat oysters dropped from 131.6 million to 65.2 million francs. Numerous companies reduced the surface... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Marteilia refringens; Crassostrea gigas; Crassostrea angulata; Ostrea edulis; Huître; Pathologie. |
| Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-5924.PDF |
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| Davenel, Armel; Gonzalez, Ricardo; Suquet, Marc; Quellec, Stephane; Robert, Rene. |
| Previous studies have shown that magnetic resonance imaging is a very appropriate non-invasive technique for quantifying the growth of somatic and gonadic tissues and sex determination in the Pacific oyster, Crassostrea gigas. Despite a thinner gonad, harder to distinguish than that of the Pacific oyster, we showed in this study that it was possible to distinguish and quantify the development of the gonad of the European flat oyster, Ostrea edulis, using a 3D MRI sequence with a suitable finer resolution. Compared to T2-weighted images, theoretically the most appropriate for good anatomical description, T1-weighted images were more suited to gonad observation. The gonad development was quantified by the number of voxels with higher grey level. Larvae were... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Gonad development; Magnetic resonance imaging. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12508/9363.pdf |
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| Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle. |
| Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that... |
| Tipo: Text |
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf |
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| Registros recuperados: 225 | |
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